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ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Editorial Board
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Visiting Research Schoalr/Postdoctoral Associate
Department of Chemistry
State University of New York at Buffalo

Buffalo, NY, USA

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Hideharu Shintani
Research Associate Professor of Medicine
National Institute of Health Science
Kyoto University

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Executive Editor
John L. Zhou
Environmental Engineering
London South Bank University

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Executive Editor
Gregory A. Hobbs
Technical Director, Toxicology
Quest Diagnostics

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Journal Impact Factor 1.01* ; 1.665* (5 Year Journal Impact Factor)
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About the Journal

NLM ID: 101623510

Index copernicus value 84.45

On behalf of the Journal of Chromatography & Separation Techniques, as Editor-in-Chief it is my distinct honor and privilege to welcome Analytical Society to our journal. As Editor-In-Chief it is my great pleasure and honor to welcome you to the Journal of Chromatography and Separation Techniques.

The Journal covers a wide variety of specialties including pharmaceutical, environmental, food and clinical sciences, reaching out to analytical scientists worldwide. The Journal emphasizes high-level research and education. Original research articles, reviews, short communications, and letters to the editors in the fields of chromatography and separation are welcome. Every effort is made to have a speedy and critical peer-review process.

I encourage you to contribute your work to the Journal and consider joining the editorial board or participate as a peer reviewer. Please feel free to contact the Journal office for any suggestions that you may have to improve the Journal.

Journal of Chromatography & Separation Techniques is at higher echelons that enhance the intelligence and information dissemination on topics closely related to Chromatography. They provide a unique forum dedicated to scientists to express their research articles, review articles, case reports and short communications on an array of Method development and Validation research.

Journal of Chromatography & Separation Techniques is an academic journal which aims to publish complete and reliable source of information on the discoveries and current developments in the mode of original articles, review articles, case reports, short communications, etc. in all areas of the field and making them freely available through online without any restrictions or any other subscriptions to researchers worldwide.

This top best scholarly journal is using Editorial tracking® System for online manuscript submission, review and tracking. Editorial board members of the Journal (or) outside experts review manuscripts; at least two independent reviewer’s approval followed by the editor is required for the acceptance of any citable manuscript.

Analytical Chromatography

Analytical Chromatography Journals has become a cornerstone of separation science, that branch of chemistry dedicated to separating compounds from mixtures. Analytical chromatography uses little sample sizes; the target is to separate compounds so as to spot them The mobile phase of chromatography refers to the fluid that carries the mixture of substances in the sample through the adsorptive material. The stationary or adsorbent phase refers to the solid material that takes up the particles of the substance passing through it. Kaolin, alumina, silica, and activated charcoal have been used as adsorbing substances or stationary phases.

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Biomedical Chromatography

Biomedical Chromatography is a process in which a chemical mixture carried by a liquid or gas is separated into components as a result of differential distribution of the solutes as they flow around or over a stationary liquid or solid phase. There are two main categories of chromatography: preparative and analytical. A sample to be separated, when placed on the stationary section, will gradually move along in the same direction as the mobile phase. If a sample compound (or analyte) has no interaction with the stationary phase, it will run right through and come out of the system (elute) at the same rate as the mobile section. On the opposite hand, if an analyte has no interaction with the mobile phase, it will stick on to the stationary phase and never elute. Neither of these are good outcomes.

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Capillary Electrochromatography

Capillary electrochromatography (CEC) is a chromatographic technique in which the mobile phase is driven through the chromatographic bed by electroosmosis. Capillary electrochromatography is a combination of two analytical techniques, High performance liquid chromatography and capillary electrophoresis. Capillary electrophoresis aims to separate analytes on the basis of their mass to charge ratio by passing a high voltage across ends of a capillary tube, which is filled with the analyte. High performance liquid chromatography separates analytes by passing them, under high pressure, through a column filled with stationary phase. Although Capillary Zone Electrophoresis (CZE) is a high-efficiency separation technique for charged analytes, it is incapable in its native form of separating neutral molecules.

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Chromatography involves a sample (or sample extract) being dissolved in a mobile phase (which may be a gas, a liquid or a supercritical fluid). The mobile phase is then forced through an immobile, immiscible stationary phase. The phases are chosen such that components of the sample have differing solubilities in each phase. A component which is quite soluble in the stationary phase will take longer to travel through it than a component which is not very soluble in the stationary phase but very soluble in the mobile phase. As a result of these differences in mobilities, sample components will become separated from each other as they travel through the stationary phase. Chromatography is a very special separation process for a multitude of reasons chromatography can be used to separate delicate products since the conditions under which it is performed are not typically severe.

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Electrophoresis is defined as the movement of charged particles in a fluid or gel under the influence of an electric field. Electrophoresis is a separations technique that is based on the mobility of ions in an electric field.
Positively charged ions migrate towards a negative conductor and negatively-charged ions migrate toward a positive conductor. For safety reasons one electrode is usually at ground and the other is biased positively or negatively. Ions have different migration rates depending on their total charge, size, and shape, and can therefore be separated. Instrumentation An electrode apparatus consists of a high-voltage supply, electrodes, buffer, and a support for the buffer such as filter paper, cellulose acetate strips, polyacrylamide gel, or a capillary tube. Open capillary tubes are used for many types of samples and the other supports are usually used for biological samples such as protein mixtures or DNA fragments. After a separation is completed the support is stained to visualize the separated components.

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Extraction Chromatography

Liquid–liquid extraction also known as solvent extraction Chromatography  and partitioning, is a method to separate compounds based on their relative solubilities in two different immiscible liquids, usually water and an organic solvent. It is an extraction of a substance from one liquid into another liquid phase. Various Industrial applications of Extraction Journals includes: Fermentation and Algae Broths, Phenol from Wastewater, Acetic Acid Extraction, Essential Oil Extraction, Caprolactam Extraction, Neutralization/washing of acids or bases from organic stream.

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Filtration is one of the process of separation and it defined as the action or process of filtering something. The act or process of filtering, especially the process of passing a liquid or gas, such as air, through a filter in order to remove solid particles. In liquid filtration Journals, such as that applied in waste material treatment, a liquid can be pulled through the filter by attraction, as in the examples given. On the opposite hand, some sort of applied pressure, or a pressure differential created by the existence of a vacuum, can force the liquid through the filter. Gas filtration is employed in a common appliance, the vacuum cleaner, which passes a stream of dust-filled air through a filtering bag inside the machine. The bag traps solid particles, while allowing clean air to pass back out into the room. This is essentially the same principle applied in air filters and even air conditioning and heating systems, which, in addition to regulating temperature, also remove dust, pollen, and other impurities from the air.

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Applications of GC-MS: Gas Chromatography and Mass-Spectroscopy Journals embrace drug detection, hearth investigation, environmental analysis, explosives investigation, and identification of unknown samples. GC-MS:Gas Chromatography and Mass-Spectroscopy Journals also can be employed in flying field security to discover substances in baggage. it will determine trace components in materials that were antecedent thought to own disintegrated on the far side identification.

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Gel Permeation Chromatography

Gel permeation chromatography, referred to as gel filtration chromatography or size exclusion chromatography, entails the chromatographic fractionation of macromolecules according to molecular size. The technique is often used for the analysis of polymers, proteins, fats, and sugars from relatively low molecular weight compounds such as pesticides in animal and plant matrices.

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HPLC Journals (High Performance Liquid chromatography Journals) has been used for medical (e.g. sleuthing cholecarciferol levels in blood serum), legal (e.g. sleuthing performance improvement medicine in urine), analysis (e.g. separating the parts of a fancy biological sample, or of comparable artificial chemicals from every other), and producing (e.g. throughout the assembly method of pharmaceutical and biological products) functions. Chromatography natural method will be delineating as a mass transfer process involving surface assimilation.

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HPTLC: High performance Thin Layer Chromatography Journals is an increased kind of Thin-layer chromatography. Variety of enhancements is often created to the fundamental technique of Thin-layer chromatography to alter the various steps, to extend the resolution achieved and to permit additional correct quantitative measurements. Automation is beneficial to beat the uncertainty in driblet size and position once the sample is applied to the attention plate by hand. One recent approach to automation has been the utilization of electricity devices and inkjet printers for applying the sample. HPTLC system as a flexible trendy analytical technique with regard to its glorious automation, improvement, flat applications. It deals with fundamentals, principle; theory of HPTLC High performance Thin Layer Chromatography Journals primarily based separation, experimental details, handling understanding and implementation.

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Immuno Affinity Chromatography

Immuno Affinity Chromatography Journals action could be a methodology of separating organic chemistry mixtures supported a extremely specific interaction like that between substance and protein, accelerator and substrate, or receptor and substance. The stationary part is often a gel matrix, usually of agarose; a linear sugar molecule derived from alga. Typically the place to begin is Associate in nursing undefined heterogeneous cluster of molecules in answer, like a cell lysate, growth medium or serum. The molecule of interest can have a accepted and outlined property, and might be exploited throughout the affinity purification method. The method itself is thought of as Associate in nursing demurrer, with the target molecule turning into at bay on a solid or stationary part or medium. The opposite molecules within the mobile part won't become at bay as they are doing not possess this property. The stationary part will then be far away from the mixture, washed and also the target molecule free from the demurrer in an exceedingly method referred to as extraction. Presumably the foremost common use of affinity action is for the purification of recombinant proteins.

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Ion-exchange chromatography

Ion-exchange chromatography retains analyte molecules on the column based on coulombic (ionic) interactions. The stationary phase surface displays ionic functional groups (R-X) that interact with analyte ions of opposite charge. This type of chromatography is further subdivided into cation exchange chromatography and anion-exchange chromatography. The ionic compound consisting of the cationic species M+ and the anionic species B- can be retained by the stationary phase. The most popular method for the purification of proteins and other charged molecules is ion exchange chromatography. In cation exchange chromatography positively charged molecules are attracted to a negatively charged solid support. Conversely, in anion exchange chromatography, negatively charged molecules are attracted to a positively charged solid support.

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Liquid chromatography–mass spectroscopy Journals (LC-MS, or alternative HPLC-MS) is an analytical chemistry technique that mixes the physical separation capabilities of liquid action (or HPLC) with the mass analysis capabilities of mass spectrographic analysis (MS). Its application is familiarised towards the separation, general detection and potential identification of chemicals of specific lots within the presence of alternative chemicals, it applications include: materia medica, Proteomics/metabolomics, Drug development.

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Mass Spectrometry Analysis in Biomolecules

This is the only analytical technique which resemblance to electrophoretic, chromatographic or ultracentrifugation methods. Mass spectrometry is an indispensable field for analyzing biomolecules like Analysis of Glycans, Analysis of Lipids, Analysis of Proteins and Peptides, Analysis of Oligonucleotides. Mass Spectrometry used in tandem with chromatographic and other separation techniques.

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Method Validation

Method validation is the process used to confirm that the analytical procedure employed for a specific test is suitable for its intended use. Results from Method Validation can be used to judge the quality, reliability and consistency of analytical results; it is an integral part of any good analytical practice. Typical Validation Characteristics: Accuracy, Precision, Repeatability, Intermediate, Precision, Specificity, Detection Limit, Quantitation Limit, Linearity, Range, Robustness. Method Validation Journals protocol lays out ahead of time the experimental design that will be used to establish the validity the analytical methods: Reagent, solvents, Sample, standard, solution preparation, Identify equipment to be used, Chromatographic conditions, System suitability, Calculations.

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Separation Techniques

Separation techniques are those techniques that can be used to separate two different states of matter such as liquid and solid. Such separation techniques include filtration or evaporation. Separation process, or a separation method, or simply a separation, is a methodology to attain any mass transfer phenomenon that converts a mixture of substances into two or more distinct product mixtures.

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Super Critical Fluid Chromatography

SFC Super Critical Fluid Chromatography Journals is employed in business primarily for separation of chiral molecules, and uses similar columns as customary HPLC systems. SFC: Super Critical Fluid Chromatography Journals is currently unremarkably used for achiral separations and purifications within the pharmaceutical business. That’s used for the analysis and purification of low to moderate relative molecular mass, thermally labile molecules. It may be used for the separation of chiral compounds. Principles are just like those of high performance liquid chromatography (HPLC), but SFC: Super Critical Fluid Chromatography Journals generally utilizes carbondioxide gas because the mobile phase; so the complete action flow path should be pressurised. As a result of the critical part represents a state during which liquid and gas properties converge, critical fluid natural action is typically referred to as "convergence natural action.

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